Argininosuccinate Lyase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32752

Novus Biologicals, part of Bio-Techne
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NBP1-32752
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Mouse, Primate

Predicted:

Bovine (98%), Mouse (96%), Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Argininosuccinate Lyase Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Argininosuccinate Lyase. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Argininosuccinate Lyase Antibody

Knockout Validated: Argininosuccinate Lyase Antibody [NBP1-32752]

Knockout Validated: Argininosuccinate Lyase Antibody [NBP1-32752]

Knockout Validated: Argininosuccinate Lyase Antibody [NBP1-32752] - Wild-type (WT) and ASL knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ASL antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Argininosuccinate Lyase Antibody [NBP1-32752]

Western Blot: Argininosuccinate Lyase Antibody [NBP1-32752]

Western Blot: Argininosuccinate Lyase Antibody [NBP1-32752] - (30 ug of whole cell lysate) A: MOLT4 10% SDS PAGE diluted at 1:1000
Immunocytochemistry/ Immunofluorescence: Argininosuccinate Lyase Antibody [NBP1-32752]

Immunocytochemistry/ Immunofluorescence: Argininosuccinate Lyase Antibody [NBP1-32752]

Immunocytochemistry/Immunofluorescence: Argininosuccinate Lyase Antibody [NBP1-32752] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ASL protein stained by ASL antibody diluted at 1:500. Blue: Hoechst 33342 staining.
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Applications for Argininosuccinate Lyase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Argininosuccinate Lyase

This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Alternate Names

argininosuccinase, argininosuccinate lyase, Arginosuccinase, ASAL, EC 4.3.2.1

Entrez Gene IDs

435 (Human)

Gene Symbol

ASL

UniProt

P04424 (Human)

Additional Argininosuccinate Lyase Products

Product Documents for Argininosuccinate Lyase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Argininosuccinate Lyase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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