Argininosuccinate Lyase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99243

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Argininosuccinate Lyase Antibodies »

Product Summary for Argininosuccinate Lyase Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Argininosuccinate Lyase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Argininosuccinate Lyase Antibody

Immunoprecipitation: Argininosuccinate Lyase Antibody [NBP2-99243]

Immunoprecipitation: Argininosuccinate Lyase Antibody [NBP2-99243]

Immunoprecipitation: Argininosuccinate Lyase Antibody [NBP2-99243] - Argininosuccinate Lyase was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 ul anti-Argininosuccinate Lyase rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Argininosuccinate Lyase rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 51 kDa. Observed band size: 54 kDa

Applications for Argininosuccinate Lyase Antibody

Application
Recommended Usage

Immunoprecipitation

5-10 uL/mg of lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Argininosuccinate Lyase

Argininosuccinate Lyase encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described.

Alternate Names

argininosuccinase, argininosuccinate lyase, Arginosuccinase, ASAL, EC 4.3.2.1

Gene Symbol

ASL

Additional Argininosuccinate Lyase Products

Product Documents for Argininosuccinate Lyase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Argininosuccinate Lyase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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