ARHGAP25 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57228

Novus Biologicals, part of Bio-Techne
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NBP2-57228
NBP2-57228-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ARHGAP25 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: LYYYKDEEDTKPQGCMYLPGCTIKEIATNPEEAGKFVFEIIPASWDQNRMGQDSYVLMASSQAEMEEWVKFLRRVAGTPC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARHGAP25 Antibody

Western Blot: ARHGAP25 Antibody [NBP2-57228]

Western Blot: ARHGAP25 Antibody [NBP2-57228]

Western Blot: ARHGAP25 Antibody [NBP2-57228] - Analysis using Anti-ARHGAP25 antibody NBP2-57228 (A) shows similar pattern to independent antibody NBP1-83053 (B).
Immunocytochemistry/ Immunofluorescence: ARHGAP25 Antibody [NBP2-57228]

Immunocytochemistry/ Immunofluorescence: ARHGAP25 Antibody [NBP2-57228]

Immunocytochemistry/Immunofluorescence: ARHGAP25 Antibody [NBP2-57228] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
Immunohistochemistry-Paraffin: ARHGAP25 Antibody [NBP2-57228]

Immunohistochemistry-Paraffin: ARHGAP25 Antibody [NBP2-57228]

Immunohistochemistry-Paraffin: ARHGAP25 Antibody [NBP2-57228] - Staining of human kidney.
View 9 more images

Applications for ARHGAP25 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARHGAP25

ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM]

Alternate Names

KAIA0053, KIAA0053, Rho GTPase activating protein 25, rho GTPase-activating protein 25, Rho-type GTPase-activating protein 25

Gene Symbol

ARHGAP25

Additional ARHGAP25 Products

Product Documents for ARHGAP25 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ARHGAP25 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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