ARHGAP28 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84642

Novus Biologicals, part of Bio-Techne
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NBP1-84642
NBP1-84642-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ARHGAP28 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KQSIRDVRDIFGVSESPPRDTCGNHTNQLDGTKEERELPRVIKTSGSMPDDASLNSTTLSDASQDKEGSFAVPRSDSVAILETIP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARHGAP28 Antibody

Western Blot: ARHGAP28 Antibody [NBP1-84642]

Western Blot: ARHGAP28 Antibody [NBP1-84642]

Western Blot: ARHGAP28 Antibody [NBP1-84642] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line CACO-2
Immunocytochemistry/ Immunofluorescence: ARHGAP28 Antibody [NBP1-84642]

Immunocytochemistry/ Immunofluorescence: ARHGAP28 Antibody [NBP1-84642]

Immunocytochemistry/Immunofluorescence: ARHGAP28 Antibody [NBP1-84642] - Immunofluorescent staining of human cell line CACO-2 shows localization to cell junctions.
Immunohistochemistry-Paraffin: ARHGAP28 Antibody [NBP1-84642]

Immunohistochemistry-Paraffin: ARHGAP28 Antibody [NBP1-84642]

Immunohistochemistry-Paraffin: ARHGAP28 Antibody [NBP1-84642] - Staining of human testis shows moderate cytoplasmic and nuclear positivity in subsets of cells in seminiferus ducts.

Applications for ARHGAP28 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARHGAP28

The ARHGAP28 gene encodes for a Rho GTPase-activating protein 28 that converts them to an inactive GDP-bound state. ARHGAP28 exists in four isoforms: isoform 1 is 729 amino acids long, 82 kDA; isoform 2 is 670 amino acids long, 75 kDA; isoform 3 is 545 amino acids long, nearly 62 kDA; isoform 5 is 570 amino acids long, 64 kDA (note, there is no isoform 4). ARHGAP28 functions in signal transduction and Rho GTPase cycle and has been investigated in various diseases such as benign meningioma.

Alternate Names

DKFZp686A2038, FLJ10312, KIAA1314FLJ27160, Rho GTPase activating protein 28, rho GTPase-activating protein 28, Rho-type GTPase-activating protein 28

Gene Symbol

ARHGAP28

Additional ARHGAP28 Products

Product Documents for ARHGAP28 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARHGAP28 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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