ARHGAP4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88528

Novus Biologicals, part of Bio-Techne
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NBP1-88528
NBP1-88528-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ARHGAP4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DRQTIETEEVNKTLKATLQALLEVVASDDGDVLDSFQTSPSTESLKSTSSDPGSRQAGRRRGQQQETETFYLTKLQEYLSGRSILAKLQAKHEKLQEALQRGDKEEQEVSWTQYT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (88%). Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARHGAP4 Antibody

Immunocytochemistry/ Immunofluorescence: ARHGAP4 Antibody [NBP1-88528]

Immunocytochemistry/ Immunofluorescence: ARHGAP4 Antibody [NBP1-88528]

Immunocytochemistry/Immunofluorescence: ARHGAP4 Antibody [NBP1-88528] - Staining of human cell line U-2 OS shows positivity in cytoplasm, focal adhesion sites and nucleus but excluded from the nucleoli.
Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88528]

Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88528]

Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88528] - Staining of human colon.
Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88528]

Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88528]

Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88528] - Staining of human cerebral cortex shows low expression as expected.
View 6 more images

Applications for ARHGAP4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARHGAP4

The ARHGAP4 gene encodes a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-bindingproteins belonging to the RAS superfamily. The protein encoded by the orthologous gene in rat is localized to theGolgi complex and can redi

Alternate Names

RGC1, Rho GTPase activating protein 4

Entrez Gene IDs

393 (Human)

Gene Symbol

ARHGAP4

UniProt

P98171 (Human)

Additional ARHGAP4 Products

Product Documents for ARHGAP4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARHGAP4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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