ARHGEF4 Antibody (ARHGEF-08)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62217

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow (Intracellular), Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # ARHGEF-08

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ARHGEF4 Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human ARHGEF4 (amino acids 143-271)

Specificity

The antibody MEM-174 recognizes CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARHGEF4 Antibody (ARHGEF-08)

Western Blot: ARHGEF4 Antibody (ARHGEF-08) [NBP2-62217]

Western Blot: ARHGEF4 Antibody (ARHGEF-08) [NBP2-62217]

Western Blot: ARHGEF4 Antibody (ARHGEF-08) [NBP2-62217] - Analysis of ARHGEF4 in HEK293 cells (A) and HEK293-ARHGEF4 transfectants (B) using mouse monoclonal anti-ARHGEF4 (clone ARHGEF-08).
Flow (Intracellular): ARHGEF4 Antibody (ARHGEF-08) [NBP2-62217]

Flow (Intracellular): ARHGEF4 Antibody (ARHGEF-08) [NBP2-62217]

Flow (Intracellular): ARHGEF4 Antibody (ARHGEF-08) [NBP2-62217] - Intracellular staining of ARHGEF4 in transfected HEK-293 cells using mouse monoclonal anti-ARHGEF4 (clone ARHGEF-08) PE.

Applications for ARHGEF4 Antibody (ARHGEF-08)

Application
Recommended Usage

Flow (Intracellular)

1-4 ug/ml

Flow Cytometry

1-4 ug/ml

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ARHGEF4

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through Gucleotide exchange protein coupled receptors. Acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. This protein is similar to rat collybistin protein. Alternative splicing of the gene generates two transcript variants which encode different isoforms.

Alternate Names

APC-stimulated guanine nucleotide exchange factor 1, Asef, ASEF1, ASEFAPC-stimulated guanine nucleotide exchange factor, GEF4, KIAA1112, Rho guanine nucleotide exchange factor (GEF) 4, rho guanine nucleotide exchange factor 4, STM6

Gene Symbol

ARHGEF4

Additional ARHGEF4 Products

Product Documents for ARHGEF4 Antibody (ARHGEF-08)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARHGEF4 Antibody (ARHGEF-08)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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