ARHGEF4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1316

Novus Biologicals, part of Bio-Techne
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NB100-1316
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ARHGEF4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence PWEEPAGEKPSCS corresponding to N-Terminus according to NP_056135.2, NP_127462.1.

Specificity

This antibody is expected to recognise both reported isoforms (NP_056135.2; NP_127462.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARHGEF4 Antibody

Western Blot: ARHGEF4 Antibody [NB100-1316]

Western Blot: ARHGEF4 Antibody [NB100-1316]

Western Blot: ARHGEF4 Antibody [NB100-1316] - Analysis of ARHGEF4 in Human Brain lysate using this antibody at 2 ug/ ml.
Western Blot: ARHGEF4 Antibody [NB100-1316]

Western Blot: ARHGEF4 Antibody [NB100-1316]

Western Blot: ARHGEF4 Antibody [NB100-1316] - HEK293 overexpressing ARHGEF4 (RC215591) and probed with this antibody (mock transfection in first lane).

Applications for ARHGEF4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

2 - 4 ug/ml
Application Notes
WB: Approx. 80 kDa band observed in human brain lysates and in transfected HEK293 transiently expressing ARHGEF4 (calculated MW of 79.1 kDa band according to NP_056135.2 and 76.4 kDa band according to 127462.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARHGEF4

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through Gucleotide exchange protein coupled receptors. Acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. This protein is similar to rat collybistin protein. Alternative splicing of the gene generates two transcript variants which encode different isoforms.

Alternate Names

APC-stimulated guanine nucleotide exchange factor 1, Asef, ASEF1, ASEFAPC-stimulated guanine nucleotide exchange factor, GEF4, KIAA1112, Rho guanine nucleotide exchange factor (GEF) 4, rho guanine nucleotide exchange factor 4, STM6

Entrez Gene IDs

50649 (Human)

Gene Symbol

ARHGEF4

UniProt

Q9NR80 (Human)

Additional ARHGEF4 Products

Product Documents for ARHGEF4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARHGEF4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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