ARHGEF6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89124

Novus Biologicals, part of Bio-Techne
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NBP1-89124
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ARHGEF6 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VQYGACEEKEERYLMLFSNVLIMLSASPRMSGFIYQGKIPIAGTVVTRLDEIEGNDCTFEITGNTVERIVVHCNNNQDFQEWLEQLNRLIRGPASCSSLSKTSSSSCSAHS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (88%). Reactivity reported in scientific literature (PMID: 26177020)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARHGEF6 Antibody

Western Blot: ARHGEF6 Antibody [NBP1-89124]

Western Blot: ARHGEF6 Antibody [NBP1-89124]

Western Blot: ARHGEF6 Antibody [NBP1-89124] - Analysis in human cell line HL-60.
Immunocytochemistry/ Immunofluorescence: ARHGEF6 Antibody [NBP1-89124]

Immunocytochemistry/ Immunofluorescence: ARHGEF6 Antibody [NBP1-89124]

Immunocytochemistry/Immunofluorescence: ARHGEF6 Antibody [NBP1-89124] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: ARHGEF6 Antibody [NBP1-89124]

Immunohistochemistry-Paraffin: ARHGEF6 Antibody [NBP1-89124]

Immunohistochemistry-Paraffin: ARHGEF6 Antibody [NBP1-89124] - Staining of human tonsil shows moderate cytoplasmic positivity in non-germinal center cells.
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Applications for ARHGEF6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARHGEF6

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein is activated by PI3-kinase. Mutations in this gene can cause X-chromosomal non-specific mental retardation. [provided by RefSeq]

Alternate Names

alphaPIX, Alpha-Pix, COOL-2, COOL2alpha, KIAA0006Cool2, mental retardation, X-linked 46, MRX46, PAK-interacting exchange factor alpha, PIXA, Rac/Cdc42 guanine exchange factor (GEF) 6, Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6, Rac/Cdc42 guanine nucleotide exchange factor 6, rho guanine nucleotide exchange factor 6

Entrez Gene IDs

9459 (Human)

Gene Symbol

ARHGEF6

UniProt

Q15052 (Human)

Additional ARHGEF6 Products

Product Documents for ARHGEF6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARHGEF6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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