ARID1A Antibody (ARID1A/7735) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27036

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # ARID1A/7735

Format

Azide and BSA Free

Concentration

1 mg/ml

View all ARID1A Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa1900-2000) of human ARID1A protein (exact sequence is proprietary)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for ARID1A Antibody (ARID1A/7735) - Azide and BSA Free

ARID1A Antibody (ARID1A/7735)

Immunohistochemistry-Paraffin: ARID1A Antibody (ARID1A/7735) - Azide and BSA Free [NBP3-27036] -

Formalin-fixed, paraffin-embedded human ovarian cancer stained with ARID1A Antibody (ARID1A/7735) - Azide and BSA Free. Inset: PBS instead of primary antibody; secondary only negative control.

Applications for ARID1A Antibody (ARID1A/7735) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml for 30 minutes at RT
Application Notes
Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: ARID1A

ARID1A encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain ARID1A genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ARID domain-containing protein 1A, AT rich interactive domain 1A (SWI- like), AT rich interactive domain 1A (SWI-like), AT-rich interactive domain-containing protein 1A, B120SWI-like protein, BAF250a, BAF250SWI/SNF complex protein p270, BM029, brain protein 120, BRG1-associated factor 250, BRG1-associated factor 250a, C10rf4, C1orf4SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatinsubfamily F member 1, chromatin remodeling factor p250, hELD, hOSA1, matrix associated, actin dependent regulator of chromatin, Osa homolog 1, OSA1, OSA1 nuclear protein, P270, SMARCF1, subfamily f, member 1

Gene Symbol

ARID1A

Additional ARID1A Products

Product Documents for ARID1A Antibody (ARID1A/7735) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARID1A Antibody (ARID1A/7735) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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