ARID1A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81795

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ARID1A Antibodies »

Product Summary for ARID1A Antibody - BSA Free

Immunogen

ARID1A antibody was raised against an 18 amino acid peptide near the amino terminus of human ARID1A. The immunogen is located within amino acids 580 - 630 of ARID1A. Amino Acid Squence: RFPPPQELSQDSFGSQAS

Specificity

ARID1A antibody is human, mouse and rat reactive. Multiple isoforms of ARID1A protein are known to exist.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

130, 260 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARID1A Antibody - BSA Free

Western Blot: ARID1A AntibodyBSA Free [NBP2-81795]

Western Blot: ARID1A AntibodyBSA Free [NBP2-81795]

Western Blot: ARID1A Antibody [NBP2-81795] - Analysis of ARID1A in SK-N-SH cell lysate with ARID1A antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: ARID1A Antibody - BSA Free [NBP2-81795]

Immunocytochemistry/ Immunofluorescence: ARID1A Antibody - BSA Free [NBP2-81795]

Immunocytochemistry/Immunofluorescence: ARID1A Antibody [NBP2-81795] - Immunofluorescence of ARID1A in mouse brain tissue with ARID1A antibody at 20 ug/ml.
Immunohistochemistry: ARID1A Antibody - BSA Free [NBP2-81795]

Immunohistochemistry: ARID1A Antibody - BSA Free [NBP2-81795]

Immunohistochemistry: ARID1A Antibody [NBP2-81795] - Immunohistochemistry of ARID1A in mouse brain tissue with ARID1A antibody at 5 ug/mL.
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Applications for ARID1A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Western Blot

1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARID1A

ARID1A encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain ARID1A genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ARID domain-containing protein 1A, AT rich interactive domain 1A (SWI- like), AT rich interactive domain 1A (SWI-like), AT-rich interactive domain-containing protein 1A, B120SWI-like protein, BAF250a, BAF250SWI/SNF complex protein p270, BM029, brain protein 120, BRG1-associated factor 250, BRG1-associated factor 250a, C10rf4, C1orf4SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatinsubfamily F member 1, chromatin remodeling factor p250, hELD, hOSA1, matrix associated, actin dependent regulator of chromatin, Osa homolog 1, OSA1, OSA1 nuclear protein, P270, SMARCF1, subfamily f, member 1

Gene Symbol

ARID1A

Additional ARID1A Products

Product Documents for ARID1A Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ARID1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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