Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ARID2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1600 and 1650 of human AT rich interactive domain 2 using the numbering given in entry NP_689854.2 (GeneID 196528).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARID2 Antibody

Western Blot: ARID2 Antibody [NBP1-26614]

Western Blot: ARID2 Antibody [NBP1-26614]

Western Blot: ARID2 Antibody [NBP1-26614] - Detection of human ARID2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID2 antibody NBP1-26614 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: ARID2 Antibody [NBP1-26614]

Immunoprecipitation: ARID2 Antibody [NBP1-26614]

Immunoprecipitation: ARID2 Antibody [NBP1-26614] - Detection of human ARID2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID2 antibody NBP1-26614 (lot NBP1-26614-2) used for IP at 6 ug per reaction. ARID2 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-26614-1) and rabbit anti-ARID2 antibody NBP1-26615. For blotting immunoprecipitated ARID2, NBP1-26614 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for ARID2 Antibody

Application
Recommended Usage

Immunoprecipitation

5-10 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ARID2

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SWI/SNF complexes possess ATPase and DNA helicase activity that promotes transcriptional activation of genes normally repressed by chromatin structure. ARID2 (AT-rich interactive domain-containing protein 2) is also known as BAF200 and was purified as a factor that associated with PBAF (hSWI/SNF-B). ARID2 is a noncatalytic subunit that is a member of the ARID (AT-rich interactive domain-containing protein) family of DNA binding proteins. In addition to the ARID domain, ARID2 also contains a C2H2-type zinc finger and has been implicated as a transcriptional co-activator for serum response factor for the regulation of cardiac genes.

Alternate Names

ARID domain-containing protein 2, AT rich interactive domain 2 (ARID, RFX-like), BAF200KIAA1557AT-rich interactive domain-containing protein 2, BRG1-associated factor 200, DKFZp686G052, DKFZp779P0222, FLJ30619, p200, Zinc finger protein with activation potential, zipzap, Zipzap/p200

Entrez Gene IDs

196528 (Human)

Gene Symbol

ARID2

UniProt

NP_689854.2 (Human)

Additional ARID2 Products

Product Documents for ARID2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ARID2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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