Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ARID2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1785 and 1835 of human AT rich interactive domain 2 using the numbering given in entry NP_689854.2 (GeneID 196528).

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARID2 Antibody

Western Blot: ARID2 Antibody [NBP1-26615]

Western Blot: ARID2 Antibody [NBP1-26615]

Western Blot: ARID2 Antibody [NBP1-26615] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Immunohistochemistry-Paraffin: ARID2 Antibody [NBP1-26615]

Immunohistochemistry-Paraffin: ARID2 Antibody [NBP1-26615]

Immunohistochemistry-Paraffin: ARID2 Antibody [NBP1-26615] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARID2 used at a dilution of 1:5,000 ( 0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: ARID2 Antibody [NBP1-26615]

Immunohistochemistry-Paraffin: ARID2 Antibody [NBP1-26615]

Immunohistochemistry-Paraffin: ARID2 Antibody [NBP1-26615] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ARID2 used at a dilution of 1:5,000 ( 0.2ug/ml). Detection: DAB
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Applications for ARID2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

5-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-26615 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ARID2

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SWI/SNF complexes possess ATPase and DNA helicase activity that promotes transcriptional activation of genes normally repressed by chromatin structure. ARID2 (AT-rich interactive domain-containing protein 2) is also known as BAF200 and was purified as a factor that associated with PBAF (hSWI/SNF-B). ARID2 is a noncatalytic subunit that is a member of the ARID (AT-rich interactive domain-containing protein) family of DNA binding proteins. In addition to the ARID domain, ARID2 also contains a C2H2-type zinc finger and has been implicated as a transcriptional co-activator for serum response factor for the regulation of cardiac genes.

Alternate Names

ARID domain-containing protein 2, AT rich interactive domain 2 (ARID, RFX-like), BAF200KIAA1557AT-rich interactive domain-containing protein 2, BRG1-associated factor 200, DKFZp686G052, DKFZp779P0222, FLJ30619, p200, Zinc finger protein with activation potential, zipzap, Zipzap/p200

Entrez Gene IDs

196528 (Human)

Gene Symbol

ARID2

UniProt

NP_689854.2 (Human)

Additional ARID2 Products

Product Documents for ARID2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ARID2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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