ARID5A Antibody (1683)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43676

Novus Biologicals, part of Bio-Techne
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NBP2-43676
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1683

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ARID5A Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARID5A. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Bovine (88%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARID5A Antibody (1683)

Western Blot: ARID5A Antibody (1683) [NBP2-43676]

Western Blot: ARID5A Antibody (1683) [NBP2-43676]

Western Blot: ARID5A Antibody (1683) [NBP2-43676] - THP-1 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody [1683]diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: ARID5A Antibody (1683) [NBP2-43676]

Immunohistochemistry-Paraffin: ARID5A Antibody (1683) [NBP2-43676]

Immunohistochemistry-Paraffin: ARID5A Antibody (1683) [NBP2-43676] - Mouse urinary bladder. ARID5A stained by ARID5A antibody [1683] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: ARID5A Antibody (1683) [NBP2-43676]

Western Blot: ARID5A Antibody (1683) [NBP2-43676]

Western Blot: ARID5A Antibody (1683) [NBP2-43676] - Analysis of Non-transfected (-) and ARID5A -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody diluted by 1:10000.
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Applications for ARID5A Antibody (1683)

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARID5A

Members of the ARID protein family, including ARID5A, have diverse functions but all appear to play important roles indevelopment, tissue-specific gene expression, and regulation of cell growth (Patsialou et al., 2005 (PubMed15640446)).(supplied by OMIM)

Alternate Names

ARID domain-containing protein 5A, AT rich interactive domain 5A (MRF1-like), AT-rich interactive domain-containing protein 5A, RFVG5814

Gene Symbol

ARID5A

Additional ARID5A Products

Product Documents for ARID5A Antibody (1683)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARID5A Antibody (1683)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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