ARID5A Antibody (921)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43770
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 921
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human ARID5A. The exact sequence is proprietary.
Reactivity Notes
Mouse (83%), Bovine (88%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ARID5A Antibody (921)
![Western Blot: ARID5A Antibody (921) [NBP2-43770]](https://resources.bio-techne.com/images/products/ARID5A-Antibody-921-Western-Blot-NBP2-43770-img0002.jpg "Western Blot: ARID5A Antibody (921) [NBP2-43770]")
Western Blot: ARID5A Antibody (921) [NBP2-43770]
Western Blot: ARID5A Antibody (921) [NBP2-43770] - THP-1 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody [921] diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.![Western Blot: ARID5A Antibody (921) [NBP2-43770]](https://resources.bio-techne.com/images/products/ARID5A-Antibody-921-Western-Blot-NBP2-43770-img0001.jpg "Western Blot: ARID5A Antibody (921) [NBP2-43770]")
Western Blot: ARID5A Antibody (921) [NBP2-43770]
Western Blot: ARID5A Antibody (921) [NBP2-43770] - Analysis of Non-transfected (-) and ARID5A-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody diluted by 1:5000.
ARID5A Antibody (921) [NBP2-43770] - ARID5A Antibody (921) detects ARID5A protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: ARID5A Antibody (921) dilution: 1:20. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
Applications for ARID5A Antibody (921)
Application
Recommended Usage
Flow Cytometry
1:20-1:200
Western Blot
1:500-1:10000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ARID5A
Alternate Names
ARID domain-containing protein 5A, AT rich interactive domain 5A (MRF1-like), AT-rich interactive domain-containing protein 5A, RFVG5814
Gene Symbol
ARID5A
Additional ARID5A Products
Product Documents for ARID5A Antibody (921)
Product Specific Notices for ARID5A Antibody (921)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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