ARID5A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81037
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LVLPYVRHLKGEDDKPLPTSKPRKQYKMAKENRGDDGATERPKKAKEERRMDQMMPGKTKADAADPAPLPSQEPPRNSTEQQGLASGSSVSFVGASGCPEAYKRLLSSFYCKGTH
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ARID5A Antibody
Western Blot: ARID5A Antibody [NBP1-81037]
Western Blot: ARID5A Antibody [NBP1-81037] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4.Immunocytochemistry/ Immunofluorescence: ARID5A Antibody [NBP1-81037]
Immunocytochemistry/Immunofluorescence: ARID5A Antibody [NBP1-81037] - Staining of human cell line A-431 shows positivity in nucleus and nucleoli.Immunohistochemistry-Paraffin: ARID5A Antibody [NBP1-81037]
Immunohistochemistry-Paraffin: ARID5A Antibody [NBP1-81037] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.Applications for ARID5A Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ARID5A
Alternate Names
ARID domain-containing protein 5A, AT rich interactive domain 5A (MRF1-like), AT-rich interactive domain-containing protein 5A, RFVG5814
Entrez Gene IDs
10865 (Human)
Gene Symbol
ARID5A
UniProt
Additional ARID5A Products
Product Documents for ARID5A Antibody
Product Specific Notices for ARID5A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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