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ARL5B Antibody (OTI2D7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01669

Novus Biologicals, part of Bio-Techne
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NBP2-01669
Conjugate
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2D7

Concentration

0.62 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human ARL5B(NP_848930) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

20.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARL5B Antibody (OTI2D7)

Western Blot: ARL5B Antibody (OTI2D7) [NBP2-01669]

Western Blot: ARL5B Antibody (OTI2D7) [NBP2-01669]

Western Blot: ARL5B Antibody (2D7) [NBP2-01669] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL5B.
Immunocytochemistry/Immunofluorescence: ARL5B Antibody (2D7) [NBP2-01669] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ARL5B.
Immunohistochemistry-Paraffin: ARL5B Antibody (OTI2D7) [NBP2-01669]

Immunohistochemistry-Paraffin: ARL5B Antibody (OTI2D7) [NBP2-01669]

Immunohistochemistry-Paraffin: ARL5B Antibody (2D7) [NBP2-01669] - Staining of paraffin-embedded Human colon tissue using anti-ARL5B mouse monoclonal antibody.

Applications for ARL5B Antibody (OTI2D7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.62 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARL5B

ARL5B (ARL8) belongs to a family of proteins that are structurally similar to ADP-ribosylation factors (ARFs; see MIM 103180). ARLs and ARFs are part of the RAS superfamily of regulatory GTPases.[supplied by OMIM].

Alternate Names

ADP-ribosylation factor-like 5B, ADP-ribosylation factor-like 8, ADP-ribosylation factor-like protein 5B, ADP-ribosylation factor-like protein 8, ADP-ribosylation-like factor 8, ARL8

Entrez Gene IDs

221079 (Human)

Gene Symbol

ARL5B

UniProt

Additional ARL5B Products

Product Documents for ARL5B Antibody (OTI2D7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ARL5B Antibody (OTI2D7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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