Aromatase Antibody (1H1)

Immunohistochemistry: Aromatase Antibody (1H1) [NBP3-26636] -

Immunohistochemistry: Aromatase Antibody (1H1) [NBP3-26636] - Image of Aromatase Antibody (1H1) diluted at 1:230 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot: Aromatase Antibody (1H1) [NBP3-26636] -

Western Blot: Aromatase Antibody (1H1) [NBP3-26636] - Positive Western Blot detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, SH-SY5Y whole cell lysate, U87 whole cell lysate, K562 whole cell lysate.
All lanes: Aromatase Antibody at 2.3ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 58, 25 KDa
Observed band size: 58 KDa

Immunocytochemistry/Immunofluorescence: Aromatase Antibody (1H1) [NBP3-26636] -

Immunocytochemistry/Immunofluorescence: Aromatase Antibody (1H1) [NBP3-26636] - Staining of HepG2 cells with Aromatase Antibody (1H1) at 1:76, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).