Aromatase Antibody (CYP19A1/4257)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07826
![Immunohistochemistry-Paraffin: Aromatase Antibody (CYP19A1/4257) [NBP3-07826]](https://resources.bio-techne.com/images/products/Aromatase-Antibody-CYP19A1-4257-Immunohistochemistry-Paraffin-NBP3-07826-img0001.jpg "Immunohistochemistry-Paraffin: Aromatase Antibody (CYP19A1/4257) [NBP3-07826]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CYP19A1/4257
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Synthetic peptide corresponding to aa376-390 of human Aromatase (KALEDDVIGYPVKK) (Uniprot: O14793)
Localization
Membrane
Specificity
CYP450 Aromatase is part of the CYP19A1 family involved in the aromatization of androgens to estrogens, a highly conserved mechanism amongst mammals. Clone CYP19A1/4257 recognizes a conserved epitope on the CYP450 aromatase, allowing for detection of aromatase levels using various analysis methods.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08711)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Aromatase Antibody (CYP19A1/4257)
Immunohistochemistry-Paraffin: Aromatase Antibody (CYP19A1/4257) [NBP3-07826]
Immunohistochemistry-Paraffin: Aromatase Antibody (CYP19A1/4257) [NBP3-07826] - Formalin-fixed, paraffin-embedded human placenta stained with Aromatase Mouse Monoclonal Antibody (CYP19A1/4257).Applications for Aromatase Antibody (CYP19A1/4257)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Aromatase
Aromatase is localized in the endoplasmic reticulum of the cell and its activity is regulated by tissue specific promoters that are in turn controlled by hormones, cytokines, and other factors. It can be found in many tissues reproductive tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, endometrium as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer.
Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function. The activity is increased by environmental factors such as age, obesity, insulin, gonadotropins, and alcohol but decreased by prolactin, anti-m llerian hormone, and smoking.
Long Name
Aromatase
Alternate Names
ARO1, CYAR, CYP19, CYP19A1, CYPXIX, EC 1.14.14.14, Estrogen synthase
Gene Symbol
CYP19A1
UniProt
Additional Aromatase Products
Product Documents for Aromatase Antibody (CYP19A1/4257)
Product Specific Notices for Aromatase Antibody (CYP19A1/4257)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...