Aromatase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1596
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Primate, Rabbit
Cited:
Human, Mouse, Rat, Primate, Rabbit
Predicted:
Bovine (90%), Equine (91%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vivo assay, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to a C-terminal portion of the human Aromatase protein (between residues 400-502). [UniProt# P11511]
Reactivity Notes
Use in Rabbit reported in scientific literature (PMID:35245530). Rabbit reactivity reported in scientific literature (PMID: 25850953). Immunogen displays the following percentage of sequence identity for non-tested species: horse (91%), cow (90%), pig (83%), sheep (81%), goat (81%), and dog (81%) proteins. Rat reactivity reported in scientific literature (PMID: 29898754). Use in Mouse reported in scientific literature (PMID:32634520).
Localization
Membrane-bound
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Aromatase Antibody
Western Blot: Aromatase Antibody [NB100-1596]
Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: Aromatase Antibody [NB100-1596]
Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aromatase Antibody NB100-1596 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596]
Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 200 um (1st image) and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.Applications for Aromatase Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:200. Use reported in scientific literature (PMID 28133606)
Immunohistochemistry-Paraffin
1:200
Simple Western
reported in scientific literature (PMID 29898754)
Western Blot
1 ug/ml
Application Notes
In Western blot, a band is seen at ~55 kDa in human brain, and Immunocytochemistry/Immunofluorescence where membrane staining is observed in SH-SY-5Y cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aromatase
Long Name
Aromatase
Alternate Names
ARO1, CYAR, CYP19, CYP19A1, CYPXIX, EC 1.14.14.14, Estrogen synthase
Gene Symbol
CYP19A1
Additional Aromatase Products
Product Documents for Aromatase Antibody
Product Specific Notices for Aromatase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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