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Artemis Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77975

Novus Biologicals, part of Bio-Techne
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NBP1-77975

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Artemis Antibody

Immunogen

This affinity purified Artemis Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding an internal region of Human Artemis (DCLRE1C DNA cross-link repair 1C). (Uniprot: Q96SD1)

Specificity

This antibody detects a band of the appropriate size in a number of different cell lines. However, it does not detect a band in cell lines that do not express artemis, such as the cell line CJ (see figure) and in an hTERT line (not shown).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Artemis Antibody

Western Blot: Artemis Antibody [NBP1-77975]

Western Blot: Artemis Antibody [NBP1-77975]

Western Blot: Artemis Antibody [NBP1-77975] - Goat-anti-Artemis was used at a 1:500 dilution to detect Artemis is various cell lysates by Western blot. CJ is an Artemis deficient cell line and so no band is visible.
Immunohistochemistry: Artemis Antibody [NBP1-77975]

Immunohistochemistry: Artemis Antibody [NBP1-77975]

Immunohistochemistry: Artemis Antibody [NBP1-77975] - Used at a 1:1000 dilution to detect Artemis by immunohistochemistry in human spleen. Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen. Tissue was formalin-fixed and paraffin embedded.
Artemis Antibody

Artemis Antibody

Affinity Purified anti-Artemis antibody was used at a 1:1000 dilution to detect Artemis by immunohistochemistry in human spleen. Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.

Applications for Artemis Antibody

Application
Recommended Usage

ELISA

1:10000-1:20000

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Reactivity in other immunoassays is unknown. This antibody detects a band of approximately 90 kDa (predicted molecular weight: 78 kDa).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Artemis

Artemis has a role in T and B lymphocyte immunodeficiency and in predisposition to lymphoma through the NHEJ pathway of DNA repair. DNA-PKcs regulates Artemis by both phosphorylation and complex formation to permit enzymatic activities that are critical for the hairpin-opening step of V(D)J recombination and for the 5' and 3' overhang processing in non-homologous DNA end joining.

Alternate Names

DCLRE1C, SCIDA, SNM11, SNM1C

Gene Symbol

DCLRE1C

Additional Artemis Products

Product Documents for Artemis Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Artemis Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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