ARX Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80506
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
A synthetic peptide from amino acid region 2-50 of human ARX conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for ARX Antibody
Western Blot: ARX Antibody [NBP2-80506]
Western Blot: ARX Antibody [NBP2-80506] - WB on mouse brain lysate. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:1500 dilution incubated overnight at 4C.Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506]
Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506] - IHC-P on paraffin sections of mouse brain. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506]
Immunohistochemistry-Paraffin: ARX Antibody [NBP2-80506] - IHC-P on paraffin sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.Applications for ARX Antibody
Application
Recommended Usage
Immunohistochemistry
1:1000 - 1:2000
Immunohistochemistry-Paraffin
1:1000 - 1:2000
Western Blot
1:1000 - 1:2000
Formulation, Preparation, and Storage
Purification
Unpurified
Reconstitution
Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.
Formulation
Lyophilized from whole serum
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ARX
Long Name
Aristless-related Homeobox Protein
Alternate Names
CT121, EIEE1, ISSX, MRX29, MRX32, MRX33, MRX36, MRX38, MRX43, MRX54, MRX76, MRX87, MRXS1, PRTS
Gene Symbol
ARX
Additional ARX Products
Product Documents for ARX Antibody
Product Specific Notices for ARX Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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