Arylsulfatase A/ARSA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00154

Novus Biologicals, part of Bio-Techne
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NBP1-00154
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Arylsulfatase A/ARSA Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-YDLSKDPGENYN corresponding to internal region according to NP_000478.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Arylsulfatase A/ARSA Antibody

Western Blot: Arylsulfatase A/ARSA Antibody [NBP1-00154]

Western Blot: Arylsulfatase A/ARSA Antibody [NBP1-00154]

Western Blot: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Western blot analysis of Mouse testes lysate (A) + peptide (B) with antibody at 1 ug/mL and Rat testes lysate (C) + peptide (D) with antibody at 0.3 ug/mL, 35ug protein in RIPA buffer. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Arylsulfatase A/ARSA Antibody [NBP1-00154]

Immunocytochemistry/ Immunofluorescence: Arylsulfatase A/ARSA Antibody [NBP1-00154]

Immunocytochemistry/Immunofluorescence: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154]

Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154]

Immunohistochemistry-Paraffin: Arylsulfatase A/ARSA Antibody [NBP1-00154] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Applications for Arylsulfatase A/ARSA Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL
Application Notes
Approx 60-65 kDa band observed in Mouse and Rat Testis lysates (calculated MW of 53.7 kDa according to Mouse NP_033843.2 and Rat NP_001030105.2;). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Arylsulfatase A/ARSA

The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Multiple alternatively spliced transcript variants, one of which encodes a distinct protein, have been described for this gene. [provided by RefSeq]

Alternate Names

ARSA, Cerebroside 3-sulfatase

Entrez Gene IDs

410 (Human); 11883 (Mouse); 315222 (Rat)

Gene Symbol

ARSA

UniProt

NP_000478.2 (Human)

Additional Arylsulfatase A/ARSA Products

Product Documents for Arylsulfatase A/ARSA Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Arylsulfatase A/ARSA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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