Arylsulfatase A/ARSA Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98210
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Mouse Arylsulfatase A/ARSA (Accession#: NP_033843.2; Met 1-Ser 506)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Arylsulfatase A/ARSA Antibody
Western Blot: Arylsulfatase A/ARSA Antibody [NBP2-98210]
Western Blot: Arylsulfatase A/ARSA Antibody [NBP2-98210] - Anti-Arylsulfatase A/ARSA rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa (We are unsure as to the identity of these extra bands).Immunoprecipitation: Arylsulfatase A/ARSA Antibody [NBP2-98210]
Immunoprecipitation: Arylsulfatase A/ARSA Antibody [NBP2-98210] - Mouse Arylsulfatase A/ARSA was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-Mouse Arylsulfatase A/ARSA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Arylsulfatase A/ARSA rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDaApplications for Arylsulfatase A/ARSA Antibody
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
4-6 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Arylsulfatase A/ARSA
Alternate Names
ARSA, Cerebroside 3-sulfatase
Gene Symbol
ARSA
Additional Arylsulfatase A/ARSA Products
Product Documents for Arylsulfatase A/ARSA Antibody
Product Specific Notices for Arylsulfatase A/ARSA Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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