ASAH1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15479
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Primate (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for ASAH1 Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ASAH1. The exact sequence is proprietary.
Reactivity Notes
Chimpanzee (100%), Bovine (87%), Rat (84%).
Localization
Lysosome
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ASAH1 Antibody
Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP2-15479]
Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP2-15479] - Immunohistochemical analysis of paraffin-embedded Adenocarcinoma, using antibody at 1:500 dilution.Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP2-15479] -
Immunohistochemistry-Paraffin: ASAH1 Antibody [NBP2-15479] - ASAH1 antibody detects ASAH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.ASAH1 stained by ASAH1 antibody (NBP2-15479) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minWestern Blot: ASAH1 Antibody [NBP2-15479] -
Western Blot: ASAH1 Antibody [NBP2-15479] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ASAH1 antibody (NBP2-15479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for ASAH1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ASAH1
Long Name
Acid ceramidase
Alternate Names
AC, ACDase, Acid CDase, ASAH, EC 3.5.1.-, EC 3.5.1.23, HSD-33, HSD33
Gene Symbol
ASAH1
UniProt
Additional ASAH1 Products
Product Documents for ASAH1 Antibody
Product Specific Notices for ASAH1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.