ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3)

Novus Biologicals, part of Bio-Techne | Catalog # H00027163-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5E3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibodies »

Product Specifications

Immunogen

NAAA (NP_055250, 36 a.a. ~ 104 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FNVSLDSVPELRWLPVLRHYDLDLVRAAMAQVIGDRVPKWVHVLIGKVVLELERFLPQPFTGEIRGMCD

Localization

Lysosome

Specificity

ASAHL - N-acylsphingosine amidohydrolase (acid ceramidase)-like

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3)

Western Blot: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3) [H00027163-M01]

Western Blot: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3) [H00027163-M01]

Western Blot: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3) [H00027163-M01] - NAAA monoclonal antibody (M01), clone 5E3 Analysis of ASAHL expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3) [H00027163-M01]

Immunocytochemistry/ Immunofluorescence: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3) [H00027163-M01]

Immunocytochemistry/Immunofluorescence: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3) [H00027163-M01] - Analysis of monoclonal antibody to NAAA on NIH/3T3 cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3) [H00027163-M01] - Detection limit for recombinant GST tagged NAAA is approximately 0.3ng/ml as a capture antibody.

Applications for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase

ASAHL (N-acylsphingosine amidohydrolase (acid ceramidase)-like) encodes an N-acylethanolamine-hydrolyzing enzyme which is highly similar to acid ceramidase. It degrades bioactive fatty acid amides to their corresponding acids and also exhibits weak hydrolytic activity against the ceramides N-lauroylsphingosine and N-palmitoylsphingosine.

Alternate Names

Acid ceramidase-like protein, ASAH-like protein, NAAA, Nacylethanolaminehydrolyzing Acid Amidase

Entrez Gene IDs

27163 (Human)

Gene Symbol

NAAA

OMIM

607469 (Human)

UniProt

NP_055250 (Human)

Additional ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Products

Product Documents for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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