ASC1 Antibody (PSH0-26)

Western Blot: ASC1 Antibody (PSH0-26) [NBP3-32049]

Western Blot: ASC1 Antibody (PSH0-26) [NBP3-32049] - Western blot analysis of ASC1 on different lysates with Rabbit anti-ASC1 antibody (NBP3-32049) at 1/1,000 dilution.

Lane 1: A549 cell lysate
Lane 2: Hela cell lysate
Lane 3: U87-MG cell lysate
Lane 4: 293T cell lysate
Lane 5: HL-60 cell lysate
Lane 6: HepG2 cell lysate
Lane 7: RH-35 cell lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 66 kDa
Observed band size: 66 kDa

Exposure time: 3 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32049) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: ASC1 Antibody (PSH0-26) [NBP3-32049]

Immunohistochemistry: ASC1 Antibody (PSH0-26) [NBP3-32049] - Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-ASC1 antibody (NBP3-32049) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32049) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.