ASCL1/Mash1 Antibody (BLR164J)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14738
Recombinant Monoclonal
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit Clone # BLR164J
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ASCL1/Mash1 Antibody (BLR164J)
Immunogen
between 186 and 236 (C-term)
Clonality
Monoclonal
Host
Rabbit
Scientific Data Images for ASCL1/Mash1 Antibody (BLR164J)
Immunoprecipitation:ASCL1/Mash1 Antibody [NBP3-14738] -
Immunoprecipitation:ASCL1/Mash1 Antibody [NBP3-14738] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from SHP-77 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiASCL1/MASH1 recombinant monoclonal antibody used for IP at 6 ul per reaction.ASCL1/MASH1 was also immunoprecipitated by a secondantibody against a different epitope of ASCL1/MASH1 For blotting immunoprecipitatedASCL1/MASH1, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin:ASCL1/Mash1 Antibody [NBP3-14738] -
Immunohistochemistry-Paraffin:ASCL1/Mash1 Antibody [NBP3-14738] -ASCL1/MASH1 recombinant monoclonal antibody [BLR164J]. Secondary: HRP-conjugated goat antirabbit IgG (A120-501P). Substrate: DAB.
Western Blot: ASCL1/Mash1 Antibody [NBP3-14738] -
Western Blot: ASCL1/Mash1 Antibody [NBP3-14738] - Whole cell lysate (50 ug) from HEK293T, HeLa, SHP-77, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ASCL1/MASH1 recombinant monoclonal antibody [BLR164J] used at 1:1000. Secondary: HRP-conjugated goat antirabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit antiActin recombinant monoclonal antibody [BLR057F].Applications for ASCL1/Mash1 Antibody (BLR164J)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 to 1:500
Immunohistochemistry
1:100 to 1:500
Immunohistochemistry-Paraffin
1:100 to 1:500
Immunoprecipitation
6 µl/1 mg lysate
Western Blot
1:1,000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
>95%
Formulation
Borate Buffered Saline (BBS) pH 8.2 with , BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: ASCL1/Mash1
Long Name
Achaete Scute Complex-like 1
Alternate Names
ASCL1, ASH1, HASH1, Mash1
Gene Symbol
ASCL1
Additional ASCL1/Mash1 Products
Product Documents for ASCL1/Mash1 Antibody (BLR164J)
Product Specific Notices for ASCL1/Mash1 Antibody (BLR164J)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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