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ASCL1/Mash1 Antibody (BLR164J)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14738

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14738

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR164J

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

between 186 and 236 (C-term)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for ASCL1/Mash1 Antibody (BLR164J)

Immunoprecipitation:ASCL1/Mash1 Antibody [NBP3-14738] -

Immunoprecipitation:ASCL1/Mash1 Antibody [NBP3-14738] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from SHP-77 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiASCL1/MASH1 recombinant monoclonal antibody used for IP at 6 ul per reaction.ASCL1/MASH1 was also immunoprecipitated by a secondantibody against a different epitope of ASCL1/MASH1 For blotting immunoprecipitatedASCL1/MASH1, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.
ASCL1/Mash1 Antibody (BLR164J)

Immunohistochemistry-Paraffin:ASCL1/Mash1 Antibody [NBP3-14738] -

Immunohistochemistry-Paraffin:ASCL1/Mash1 Antibody [NBP3-14738] -ASCL1/MASH1 recombinant monoclonal antibody [BLR164J]. Secondary: HRP-conjugated goat antirabbit IgG (A120-501P). Substrate: DAB.
ASCL1/Mash1 Antibody (BLR164J)

Western Blot: ASCL1/Mash1 Antibody [NBP3-14738] -

Western Blot: ASCL1/Mash1 Antibody [NBP3-14738] - Whole cell lysate (50 ug) from HEK293T, HeLa, SHP-77, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ASCL1/MASH1 recombinant monoclonal antibody [BLR164J] used at 1:1000. Secondary: HRP-conjugated goat antirabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit antiActin recombinant monoclonal antibody [BLR057F].

Applications for ASCL1/Mash1 Antibody (BLR164J)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 to 1:500

Immunohistochemistry

1:100 to 1:500

Immunohistochemistry-Paraffin

1:100 to 1:500

Immunoprecipitation

6 µl/1 mg lysate

Western Blot

1:1,000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with , BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: ASCL1/Mash1

MASH1 encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.

Long Name

Achaete Scute Complex-like 1

Alternate Names

ASCL1, ASH1, HASH1, Mash1

Gene Symbol

ASCL1

Additional ASCL1/Mash1 Products

Product Documents for ASCL1/Mash1 Antibody (BLR164J)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ASCL1/Mash1 Antibody (BLR164J)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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