Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3G5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ASPA Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 77-313 of human ASPA(NP_000040) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ASPA Antibody (OTI3G5) - Azide and BSA Free

Western Blot: ASPA Antibody (OTI3G5)Azide and BSA Free [NBP2-70216]

Western Blot: ASPA Antibody (OTI3G5)Azide and BSA Free [NBP2-70216]

Western Blot: ASPA Antibody (OTI3G5) - Azide and BSA Free [NBP2-70216] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASPA.
Immunohistochemistry: ASPA Antibody (OTI3G5) - Azide and BSA Free [NBP2-70216]

Immunohistochemistry: ASPA Antibody (OTI3G5) - Azide and BSA Free [NBP2-70216]

Immunohistochemistry: ASPA Antibody (OTI3G5) - Azide and BSA Free [NBP2-70216] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: ASPA Antibody (OTI3G5) - Azide and BSA Free [NBP2-70216]

Immunohistochemistry: ASPA Antibody (OTI3G5) - Azide and BSA Free [NBP2-70216]

Immunohistochemistry: ASPA Antibody (OTI3G5) - Azide and BSA Free [NBP2-70216] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
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Applications for ASPA Antibody (OTI3G5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ASPA

ASPA encodes an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues. Mutations in this gene cause Canavan disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Alternate Names

ACY-2, ACY2aminoacylase 2, Aminoacylase-2, ASPaminoacylase-2, aspartoacylase, aspartoacylase (aminoacylase 2, Canavan disease), EC 3.5.1.15

Gene Symbol

ASPA

Additional ASPA Products

Product Documents for ASPA Antibody (OTI3G5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ASPA Antibody (OTI3G5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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