ASPA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31754

Novus Biologicals, part of Bio-Techne
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NBP1-31754
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Monkey

Predicted:

Bovine (93%), Porcine (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ASPA Antibodies »

Product Summary for ASPA Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ASPA. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%).

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ASPA Antibody

Western Blot: ASPA Antibody [NBP1-31754]

Western Blot: ASPA Antibody [NBP1-31754]

Western Blot: ASPA Antibody [NBP1-31754] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Aspartoacylase antibody [N1C3-2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ASPA Antibody [NBP1-31754]

Immunocytochemistry/ Immunofluorescence: ASPA Antibody [NBP1-31754]

Immunocytochemistry/Immunofluorescence: ASPA Antibody [NBP1-31754] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aspartoacylase protein stained by partoacylase antibody [N1C3-2] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-31754]

Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-31754]

Immunohistochemistry-Paraffin: ASPA Antibody [NBP1-31754] - Cal27 xenograft, using Aspartoacylase antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Applications for ASPA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ASPA

This gene encodes an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues. Mutations in this gene cause Canavan disease. Alternatively spliced transcript variants have been found for this gene.

Alternate Names

ACY-2, ACY2aminoacylase 2, Aminoacylase-2, ASPaminoacylase-2, aspartoacylase, aspartoacylase (aminoacylase 2, Canavan disease), EC 3.5.1.15

Gene Symbol

ASPA

Additional ASPA Products

Product Documents for ASPA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ASPA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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