Asparagine synthetase Antibody (S05-9E5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19507

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19507-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S05-9E5

Concentration

0.3 mg/ml

View all Asparagine Synthetase/ASNS Antibodies »

Product Summary for Asparagine synthetase Antibody (S05-9E5)

Immunogen

A synthetic peptide of human Asparagine synthetase (Uniprot # P08243)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Asparagine synthetase Antibody (S05-9E5)

Western Blot: Asparagine synthetase Antibody (S05-9E5) [NBP3-19507]

Western Blot: Asparagine synthetase Antibody (S05-9E5) [NBP3-19507]

Western Blot: Asparagine synthetase Antibody (S05-9E5) [NBP3-19507] - Western blot detection of Asparagine synthetase in K562, Hela cell lysates using NBP3-19507 (1:1000 diluted). Predicted band size: 64kDa. Observed band size: 64kDa.
Immunohistochemistry-Paraffin: Asparagine synthetase Antibody (S05-9E5) [NBP3-19507]

Immunohistochemistry-Paraffin: Asparagine synthetase Antibody (S05-9E5) [NBP3-19507]

Immunohistochemistry-Paraffin: Asparagine synthetase Antibody (S05-9E5) [NBP3-19507] - Immunohistochemistry of Asparagine synthetase in paraffin-embedded Human tonsil using NBP3-19507 at dilution 1/100

Applications for Asparagine synthetase Antibody (S05-9E5)

Application
Recommended Usage

Immunohistochemistry

1/20

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Asparagine Synthetase/ASNS

Asparagine synthetase (AS) is a housekeeping enzyme responsible for the production of asparagine from aspartate and glutamine. Most mammalian cells express AS and regulate the level of activity in response to the concentration of asparagine in the medium. Certain tumor cells in contrast, exhibit little or no AS activity and rely on the surrounding medium as a source of exogenous asparagines (1). Therefore tumor cells may be selectively killed by a chemotherapeutic drug using asparaginase. This approach has been exploited in the treatment of certain cancers like childhood acute lymphoblastic leukemia (2).

Long Name

ASNS

Alternate Names

ASNSD, EC 6.3.5.4, Glutamine Dependent Asparagine Synthetase, TS11 Cell Cycle Control Protein

Gene Symbol

ASNS

Additional Asparagine Synthetase/ASNS Products

Product Documents for Asparagine synthetase Antibody (S05-9E5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Asparagine synthetase Antibody (S05-9E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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