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Asporin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1514

Novus Biologicals, part of Bio-Techne
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NB100-1514
NB100-1514-25ug

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-IHENKVKKIQKDT corresponding to internal region according to NP_060150.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Asporin Antibody

Western Blot: Asporin Antibody [NB100-1514]

Western Blot: Asporin Antibody [NB100-1514]

Western Blot: Asporin Antibody [NB100-1514] - (0.1ug/ml) staining of Human Tonsil (A), (0.3ug/ml) Human Uterus (B), Mouse Skeletal Muscle (C) (1ug/ml) Rat Skeletal Muscle (D) and negative control Human Cerebellum (E) lysate (35ug protein in RIPA buffer).Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Asporin Antibody [NB100-1514]

Immunocytochemistry/ Immunofluorescence: Asporin Antibody [NB100-1514]

Immunocytochemistry/Immunofluorescence: Asporin Antibody [NB100-1514] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear membrane staining. The nuclear stain is DA

Applications for Asporin Antibody

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

detection limit 1:64000

Western Blot

0.1-1 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Asporin

ASPN, or Asporin, belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin. Asporin is expressed in a variety of human tissues with higher levels in osteoarthritic articular cartilage, aorta, uterus, heart and liver. The function of Asporin is unclear, but available data indicates a collagen binding capacity.

Alternate Names

asporin, asporin (LRR class 1), FLJ20129, OS3, periodontal ligament associated protein 1, Periodontal ligament-associated protein 1, PLAP-1, PLAP1SLRR1Casporin proteoglycan, small leucine-rich protein 1C

Entrez Gene IDs

54829 (Human)

Gene Symbol

ASPN

UniProt

Additional Asporin Products

Product Documents for Asporin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Asporin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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