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Asporin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-58061

Novus Biologicals, part of Bio-Techne
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NBP1-58061

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptides corresponding to ASPN(asporin) The peptide sequence was selected from the middle region of ASPN. Peptide sequence NKISTVELEDFKRYKELQRLGLGNNKITDIENGSLANIPRVREIHLENNK. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Asporin Antibody

Western Blot: Asporin Antibody [NBP1-58061]

Western Blot: Asporin Antibody [NBP1-58061]

Western Blot: Asporin Antibody [NBP1-58061] - Sample Tissue: HepG2, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5ug/mL, Peptide Concentration: 2.0ug/mL, Lysate Quantity: 25ug/lane, Gel Concentration: 12%
Immunohistochemistry-Paraffin: Asporin Antibody [NBP1-58061]

Immunohistochemistry-Paraffin: Asporin Antibody [NBP1-58061]

Immunohistochemistry-Paraffin: Asporin Antibody [NBP1-58061] - Human Muscle Tissue, Skeletal muscle cells (Indicated with Arrows) 4-8ug/ml.
Western Blot: Asporin Antibody [NBP1-58061]

Western Blot: Asporin Antibody [NBP1-58061]

Western Blot: Asporin Antibody [NBP1-58061] - HepG2 cell lysate, Antibody Titration: 2.5ug/ml

Applications for Asporin Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Asporin

ASPN belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin.ASPN belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin (MIM 125255) (Lorenzo et al., 2001).[supplied by OMIM].

Alternate Names

asporin, asporin (LRR class 1), FLJ20129, OS3, periodontal ligament associated protein 1, Periodontal ligament-associated protein 1, PLAP-1, PLAP1SLRR1Casporin proteoglycan, small leucine-rich protein 1C

Gene Symbol

ASPN

UniProt

Additional Asporin Products

Product Documents for Asporin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Asporin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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