Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ASPP1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 475 and 525 of human Apoptosis-Stimulated Protein of p53, 1 using the numbering given in entry NP_056131.1 (GeneID 23368).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASPP1 Antibody

Western Blot: ASPP1 Antibody [NB110-40684]

Western Blot: ASPP1 Antibody [NB110-40684]

Western Blot: ASPP1 Antibody [NB110-40684] - Detection of Human ASPP1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASPP1 antibody NB110-40684 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 60 seconds.
Immunoprecipitation: ASPP1 Antibody [NB110-40684]

Immunoprecipitation: ASPP1 Antibody [NB110-40684]

Immunoprecipitation: ASPP1 Antibody [NB110-40684] - Detection of human ASPP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASPP1 antibody NB110-40684 used for IP at 6 ug per reaction. ASPP1 was also immunoprecipitated by rabbit anti-ASPP1 antibody NB110-40683. For blotting immunoprecipitated ASPP1, NB110-40684 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for ASPP1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ASPP1

The ASPP family members ASPP1, ASPP2, act as potent activators of p53. A third member of the family, iASPP, has an inhibitory effect on apoptosis. The three members of ASPP family all bind p53 via their C-terminus, which contains a proline-rich region, four ankyrin repeats and an SH3 domain. Expression of ASPP1 and ASPP2 is frequently downregulated in human breast tumors expressing wild-type p53. ASPP1 may also play a role in vascular development.

Alternate Names

apoptosis-stimulating protein of p53, 1, ASPP1Protein phosphatase 1 regulatory subunit 13B, KIAA0771apoptosis-stimulating of p53 protein 1, p53BP2-like, p85, protein phosphatase 1, regulatory (inhibitor) subunit 13B

Entrez Gene IDs

23368 (Human)

Gene Symbol

PPP1R13B

UniProt

Q96KQ4 (Human)

Additional ASPP1 Products

Product Documents for ASPP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ASPP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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