ASTE1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81661

Novus Biologicals, part of Bio-Techne
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NBP1-81661
NBP1-81661-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ASTE1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CFVQCFSEADRDIMTLANHWNCPVLSSDSDFCIFDLKTGFCPLNSFQWRNMNTIKGTQNYIPAKCFSLDAFCHHFSNMNKALLPLFAVLCGNDHVNLPIME

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASTE1 Antibody

Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]

Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]

Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661] - Staining in human epididymis and pancreas tissues using anti-ASTE1 antibody. Corresponding ASTE1 RNA-seq data are presented for the same tissues.
Western Blot: ASTE1 Antibody [NBP1-81661]

Western Blot: ASTE1 Antibody [NBP1-81661]

Western Blot: ASTE1 Antibody [NBP1-81661] - Analysis in control (vector only transfected HEK293T lysate) and ASTE1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ASTE1 Antibody [NBP1-81661]

Immunocytochemistry/ Immunofluorescence: ASTE1 Antibody [NBP1-81661]

Immunocytochemistry/Immunofluorescence: ASTE1 Antibody [NBP1-81661] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
View 2 more images

Applications for ASTE1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASTE1

ASTE1, also known as Protein asteroid homolog 1, is a 679 amino acid protein that is 77 kDa, belongs to the asteroid family, and has a possible role in EGF receptor signaling. The protein is being studied for its involvement in colorectal cancer. ASTE1 has been linked to ERBB3 in DNA repair pathway.

Alternate Names

asteroid homolog 1 (Drosophila), HT001, MGC129980, protein asteroid homolog 1

Gene Symbol

ASTE1

Additional ASTE1 Products

Product Documents for ASTE1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ASTE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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