Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Ataxin-2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1262 and 1312 of human ataxin 2 (spinocerebellar ataxia 2) using the numbering given in entry NP_002964.2 (GeneID 6311).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ataxin-2 Antibody

Western Blot: Ataxin-2 Antibody [NB100-58797]

Western Blot: Ataxin-2 Antibody [NB100-58797]

Western Blot: Ataxin-2 Antibody [NB100-58797] - Detection of Human and Mouse ATX2/SCA2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-ATX2/SCA2 antibody NB100-58797 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Ataxin-2 Antibody [NB100-58797]

Immunoprecipitation: Ataxin-2 Antibody [NB100-58797]

Immunoprecipitation: Ataxin-2 Antibody [NB100-58797] - Detection of human ATX2/SCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-ATX2/SCA2 antibody NB100-58797 used for IP at 6 ug/mg lysate. ATX2/SCA2 was also immunoprecipitated by rabbit anti-ATX2/SCA2 antibody from Company B. For blotting immunoprecipitated ATX2/SCA2, NB100-58797 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Ataxin-2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Ataxin-2

The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. This locus has been mapped to chromosome 12, and it has been determined that the diseased allele contains 36-52 CAG repeats, compared to 22-23 in the normal allele. A potential transcript variant, missing an internal coding exon, has been described; however, its full-length nature is not certain.

Alternate Names

ataxin 2, ataxin 2), ataxin-2, ATX2spinocerebellar ataxia 2 (olivopontocerebellar ataxia 2, autosomal dominant, SCA2FLJ46772, Spinocerebellar ataxia type 2 protein, TNRC13, trinucleotide repeat containing 13, Trinucleotide repeat-containing gene 13 protein

Entrez Gene IDs

6311 (Human)

Gene Symbol

ATXN2

UniProt

Q99700 (Human)

Additional Ataxin-2 Products

Product Documents for Ataxin-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ataxin-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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