Ataxin-2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76807
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-SEREGHSINTR, from the internal region of the protein sequence according to NP_002964.3; NP_001297050.1; NP_001297052.1.
Epitope
C-SEREGHSINTR
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Ataxin-2 Antibody
Immunocytochemistry/ Immunofluorescence: Ataxin-2 Antibody [NBP2-76807]
Immunocytochemistry/Immunofluorescence: Ataxin-2 Antibody [NBP2-76807] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).Flow Cytometry: Ataxin-2 Antibody [NBP2-76807]
Flow Cytometry: Ataxin-2 Antibody [NBP2-76807] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Immunocytochemistry/ Immunofluorescence: Ataxin-2 Antibody [NBP2-76807]
Immunocytochemistry/Immunofluorescence: Ataxin-2 Antibody [NBP2-76807] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).Applications for Ataxin-2 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Peptide ELISA
1:200
Application Notes
WB: Preliminary experiments in human brain (cerebellum and cerebral cortex) lysates gave no specific signal but low background.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Ataxin-2
Alternate Names
ataxin 2, ataxin 2), ataxin-2, ATX2spinocerebellar ataxia 2 (olivopontocerebellar ataxia 2, autosomal dominant, SCA2FLJ46772, Spinocerebellar ataxia type 2 protein, TNRC13, trinucleotide repeat containing 13, Trinucleotide repeat-containing gene 13 protein
Gene Symbol
ATXN2
Additional Ataxin-2 Products
Product Specific Notices for Ataxin-2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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