Ataxin 7-Like 3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99131
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Ataxin 7-Like 3.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Ataxin 7-Like 3 Antibody
Western Blot: Ataxin 7-Like 3 Antibody [NBP2-99131]
Western Blot: Ataxin 7-Like 3 Antibody [NBP2-99131] - Lane A: Jurkat Whole Cell LysateLane B: Raji Whole Cell LysateLane C: K562 Whole Cell LysateLane D: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:39 kDaObserved band size:49 kDa(We are unsure as to the identity of these extra bands.)Immunoprecipitation: Ataxin 7-Like 3 Antibody [NBP2-99131]
Immunoprecipitation: Ataxin 7-Like 3 Antibody [NBP2-99131] - Lane A:0.5 mg Raji Whole Cell LysateLane B:0.5 mg K562 Whole Cell LysateLane C:0.5 mg THP-1 Whole Cell Lysate4 uL anti-ATXN7L3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ATXN7L3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 45 kDaApplications for Ataxin 7-Like 3 Antibody
Application
Recommended Usage
Immunoprecipitation
4-8 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ataxin 7-Like 3
Alternate Names
Ataxin-7-Like Protein 3, ATXN7L3, SAGA-Associated Factor 11 Homolog
Gene Symbol
ATXN7L3
Additional Ataxin 7-Like 3 Products
Product Documents for Ataxin 7-Like 3 Antibody
Product Specific Notices for Ataxin 7-Like 3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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