ATF1 Antibody (3E7)

Novus Biologicals, part of Bio-Techne | Catalog # H00000466-M03

Novus Biologicals, part of Bio-Techne
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H00000466-M03
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3E7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATF1 Antibodies »

Product Specifications

Immunogen

ATF1 (AAH29619, 1 a.a. ~ 271 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPNGALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSNKSV

Specificity

ATF1 - activating transcription factor 1 (3E7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ATF1 Antibody (3E7)

Western Blot: ATF1 Antibody (3E7) [H00000466-M03]

Western Blot: ATF1 Antibody (3E7) [H00000466-M03]

Western Blot: ATF1 Antibody (3E7) [H00000466-M03] - Analysis of ATF1 expression in transfected 293T cell line by ATF1 monoclonal antibody (M03), clone 3E7. Lane 1: ATF1 transfected lysatE (29.2 KDa). Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: ATF1 Antibody (3E7) [H00000466-M03]

Immunohistochemistry-Paraffin: ATF1 Antibody (3E7) [H00000466-M03]

Immunohistochemistry-Paraffin: ATF1 Antibody (3E7) [H00000466-M03] - Analysis of monoclonal antibody to ATF1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml
Western Blot: ATF1 Antibody (3E7) [H00000466-M03]

Western Blot: ATF1 Antibody (3E7) [H00000466-M03]

Western Blot: ATF1 Antibody (3E7) [H00000466-M03] - Western blot analysis of ATF1 over-expressed 293 cell line. Lane 2 cotransfected with ATF1 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000466-M03. GAPDH (36.1 kDa) used as loading control.
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Applications for ATF1 Antibody (3E7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been used for RNAi Validation and IHC-P.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATF1

ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.

Long Name

Activating Transcription Factor 1

Alternate Names

TREB36

Entrez Gene IDs

466 (Human)

Gene Symbol

ATF1

UniProt

AAH29619 (Human)

Additional ATF1 Products

Product Documents for ATF1 Antibody (3E7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATF1 Antibody (3E7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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