ATF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52647

Novus Biologicals, part of Bio-Techne
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NBP1-52647
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all ATF1 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 221 and 271 of human Activating Transcription Factor 1 using the numbering given in entry NP_005162.1 (GeneID 466).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATF1 Antibody

Western Blot: ATF1 Antibody [NBP1-52647]

Western Blot: ATF1 Antibody [NBP1-52647]

Western Blot: ATF1 Antibody [NBP1-52647] - Detection of Human ATF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATF1 antibody NBP1-52647 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: ATF1 Antibody [NBP1-52647]

Western Blot: ATF1 Antibody [NBP1-52647]

Western Blot: ATF1 Antibody [NBP1-52647] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-ATF1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ATF1 was also immunoprecipitated by rabbit anti-ATF1 antibodies NBP1-52645 and NBP1-52646, which recognize upstream epitopes.

Applications for ATF1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ATF1

Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/ CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to play a key role in the induction of NOX1, this protein binds the cAMP response element (CRE) and mediates PKA-induced stimulation of CRE-reporter genes. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CREdependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylates Histones H2B and H4 specifically in vitro.

Long Name

Activating Transcription Factor 1

Alternate Names

TREB36

Gene Symbol

ATF1

Additional ATF1 Products

Product Documents for ATF1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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