ATF2 Antibody (PCRP-ATF2-1B4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13855

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-ATF2-1B4

Concentration

0.2 mg/ml

View all ATF2 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human ATF2 protein (Uniprot: P15336)

Reactivity Notes

Predicted to react in Mouse and Rat.

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

54.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14001)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for ATF2 Antibody (PCRP-ATF2-1B4)

Immunocytochemistry/ Immunofluorescence: ATF2 Antibody (PCRP-ATF2-1B4) [NBP3-13855]

Immunocytochemistry/ Immunofluorescence: ATF2 Antibody (PCRP-ATF2-1B4) [NBP3-13855]

Immunocytochemistry/Immunofluorescence: ATF2 Antibody (PCRP-ATF2-1B4) [NBP3-13855] - Immunofluorescence Analysis of HeLacells stained using ATF2 Antibody (PCRP-ATF2-1B4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometry: ATF2 Antibody (PCRP-ATF2-1B4) [NBP3-13855]

Flow Cytometry: ATF2 Antibody (PCRP-ATF2-1B4) [NBP3-13855]

Flow Cytometry: ATF2 Antibody (PCRP-ATF2-1B4) [NBP3-13855] - Flow Cytometric Analysis of PFA-fixed U87cells. ATF2 antibody (PCRP-ATF2-1B4) followed bygoat anti-mouse IgG-CF488 (blue); unstained cells (red).
Flow Cytometry: ATF2 Antibody (PCRP-ATF2-1B4) [NBP3-13855]

Flow Cytometry: ATF2 Antibody (PCRP-ATF2-1B4) [NBP3-13855]

Flow Cytometry: ATF2 Antibody (PCRP-ATF2-1B4) [NBP3-13855] - Flow Cytometric Analysis of PFA-fixed HeLacells. ATF2 antibody (PCRP-ATF2-1B4) followed bygoat anti-mouse IgG-CF488 (blue); unstained cells (red).
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Applications for ATF2 Antibody (PCRP-ATF2-1B4)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ATF2

ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Long Name

Activating Transcription Factor 2

Alternate Names

CRE-BP1, CREB2, HB16, mXBP, TREB7

Gene Symbol

ATF2

UniProt

P15336

Additional ATF2 Products

Product Documents for ATF2 Antibody (PCRP-ATF2-1B4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATF2 Antibody (PCRP-ATF2-1B4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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