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ATF2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85457

Novus Biologicals, part of Bio-Techne
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NBP1-85457-25ul
NBP1-85457

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSNVHVPAAVPLVRPVTMVPSVPGIPGPSSPQPVQSEAKMRLKAALTQQHPPVTNGDTVKGHGSGLVRTQSEESRPQSLQQPATSTTETPASPAHTTPQTQSTSGRRRRAANEDPDEKRRKFLERNRAAASRCRQKRKVWVQS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATF2 Antibody

Immunocytochemistry/ Immunofluorescence: ATF2 Antibody [NBP1-85457]

Immunocytochemistry/ Immunofluorescence: ATF2 Antibody [NBP1-85457]

Immunocytochemistry/Immunofluorescence: ATF2 Antibody [NBP1-85457] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: ATF2 Antibody [NBP1-85457]

Immunohistochemistry-Paraffin: ATF2 Antibody [NBP1-85457]

Immunohistochemistry-Paraffin: ATF2 Antibody [NBP1-85457] - Staining of human prostate shows moderate nuclear positivity in glandular cells.
ATF2 Antibody

Western Blot: ATF2 Antibody [NBP1-85457] -

Western Blot: ATF2 Antibody [NBP1-85457] - Analysis in human cell line HepG2.

Applications for ATF2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATF2

ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Long Name

Activating Transcription Factor 2

Alternate Names

CRE-BP1, CREB2, HB16, mXBP, TREB7

Gene Symbol

ATF2

Additional ATF2 Products

Product Documents for ATF2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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