ATF2 [p Thr52] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29528

Novus Biologicals, part of Bio-Techne
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NBP2-29528
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATF2 Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding THr52 conjugated to KLH. Accession # P15336

Reactivity Notes

Non-human primate. The antibody has been directly tested for reactivity in Western blots with UACC903 melanoma cells.

Modification

p Thr52

Specificity

Specific for endogenous levels of the ~72 kDa ATF2 protein phosphorylated at Thr52. Immunolabeling of the ATF2 band is reduced by treatment of the cells with the PKC catalytic inhibitor Go6850.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATF2 [p Thr52] Antibody

Western Blot: ATF2 [p Thr52] Antibody [NBP2-29528]

Western Blot: ATF2 [p Thr52] Antibody [NBP2-29528]

Western Blot: ATF2 [p Thr52] Antibody [NBP2-29528] - Western blot of UACC903 melanoma cell line lysate (DMSO) showing specific immunolabeling of the ~72k ATF2 protein phosphorylated at Thr52. Immunolabeling is reduced by treatment of the lysate with the PKC catalytic inhibitor Go6850. Image courtesy of Eric Lau and Ze'ev Ronai.

Applications for ATF2 [p Thr52] Antibody

Application
Recommended Usage

Western Blot

1:250

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATF2

The transcription factor ATF2 is a member of the ATF/CREB family of leucine zipper proteins. In response to stress stimuli, it activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype (Vlahopoulos et al., 2008). Inhibiting ATF2 impedes melanoma development and elicits tumor suppressor function (Bhoumik et al., 2008). To act as a tumor suppressor, ATF2 must localize at the mitochondria, and phosphorylation at Thr52 by PKC-epsilon regulates this translocation (Lau et al., 2012).

Long Name

Activating Transcription Factor 2

Alternate Names

CRE-BP1, CREB2, HB16, mXBP, TREB7

Entrez Gene IDs

1386 (Human)

Gene Symbol

ATF2

UniProt

P15336

Additional ATF2 Products

Product Documents for ATF2 [p Thr52] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATF2 [p Thr52] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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