Key Product Details

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # S360A-24

Concentration

1 mg/ml

View all ATF4 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 25-327 of human ATF4. 86% identical to rat, and 85% identical to mouse. <50% identity with ATF5.

Localization

Cytoplasm , Cell Membrane , Nucleus

Specificity

Less then 50% identity with ATF5.Detects approx 60kDa. Does not cross-react with ATF5.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for ATF4 Antibody (S360A-24)

Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176]

Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176]

Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176] - Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4protein using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~39 kDa (isoform 2).
Immunocytochemistry/ Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176]

Immunocytochemistry/ Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176]

Immunocytochemistry/Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) ATF4Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176]

Immunocytochemistry/ Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176]

Immunocytochemistry/Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Cell Membrane. Magnification: 60X.

Applications for ATF4 Antibody (S360A-24)

Application
Recommended Usage

Western Blot

1:1000
Application Notes
1 ug/ml of ATF4 Antibody was sufficient for detection of ATF4 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATF4

ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

Long Name

Activating Transcription Factor 4

Alternate Names

TAXREB67, TXREB

Gene Symbol

ATF4

UniProt

NP_001666.2 (Human), P18848 (Human)

Additional ATF4 Products

Product Documents for ATF4 Antibody (S360A-24)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATF4 Antibody (S360A-24)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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