ATF5 Antibody (SD2099)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67767

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67767
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SD2099

Concentration

1 mg/ml

View all ATF5 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human ATF5 aa 203-252 / 282. (SwissProt: Q9Y2D1 Human; SwissProt: O70191 Mouse; SwissProt: Q6P788 Rat)

Localization

Centrosome, Nucleus, Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATF5 Antibody (SD2099)

Western Blot: ATF5 Antibody (SD2099) [NBP2-67767]

Western Blot: ATF5 Antibody (SD2099) [NBP2-67767]

Western Blot: ATF5 Antibody (SD2099) [NBP2-67767] - Western blot analysis of ATF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb
Immunocytochemistry/ Immunofluorescence: ATF5 Antibody (SD2099) [NBP2-67767]

Immunocytochemistry/ Immunofluorescence: ATF5 Antibody (SD2099) [NBP2-67767]

Immunocytochemistry/Immunofluorescence: ATF5 Antibody (SD2099) [NBP2-67767] - Staining ATF5 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: ATF5 Antibody (SD2099) [NBP2-67767]

Immunohistochemistry-Paraffin: ATF5 Antibody (SD2099) [NBP2-67767]

Immunohistochemistry-Paraffin: ATF5 Antibody (SD2099) [NBP2-67767] - Analysis of paraffin-embedded mouse stomach tissue using anti-ATF5 antibody. Counter stained with hematoxylin.
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Applications for ATF5 Antibody (SD2099)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATF5

Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4

Alternate Names

activating transcription factor 5FLJ34666, ATFXHMFN0395, cAMP-dependent transcription factor ATF-5, cyclic AMP-dependent transcription factor ATF-5, Transcription factor ATFx

Gene Symbol

ATF5

Additional ATF5 Products

Product Documents for ATF5 Antibody (SD2099)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATF5 Antibody (SD2099)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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