ATG4B Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41307
![Western Blot: ATG4B AntibodyBSA Free [NBP2-41307]](https://resources.bio-techne.com/images/products/ATG4B-Antibody-Western-Blot-NBP2-41307-img0003.jpg "Western Blot: ATG4B AntibodyBSA Free [NBP2-41307]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Predicted:
Bovine (94%). Backed by our 100% Guarantee.
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ATG4B. The immunogen is located within the last 50 amino acids of ATG4B. Amino Acid Squence: LDSSDVERLERFFDSED
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (88%).
Specificity
At least two isoforms of ATG4B are known to exist; this antibody will detect only the larger isoform. ATG4B is predicted to not cross-react with other ATG4 proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ATG4B Antibody - BSA Free
Western Blot: ATG4B AntibodyBSA Free [NBP2-41307]
Western Blot: ATG4B Antibody [NBP2-41307] - Analysis of ATG4B in (A) Ramos and (B) Jurkat cell lysate with ATG4B antibody at 1 ug/mL.![Immunohistochemistry-Paraffin: ATG4B Antibody - BSA Free [NBP2-41307]](https://resources.bio-techne.com/images/products/ATG4B-Antibody-Immunohistochemistry-Paraffin-NBP2-41307-img0001.jpg "Immunohistochemistry-Paraffin: ATG4B Antibody - BSA Free [NBP2-41307]")
Immunohistochemistry-Paraffin: ATG4B Antibody - BSA Free [NBP2-41307]
Immunohistochemistry-Paraffin: ATG4B Antibody [NBP2-41307] - Human spleen tissue with ATG4B antibody at 5 ug/ml.
Immunohistochemistry: ATG4B Antibody [NBP2-41307] -
Immunohistochemistry: ATG4B Antibody [NBP2-41307] - Human spleen tissue with ATG4B antibody at 20 ug/ml.Applications for ATG4B Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ATG4B
Long Name
ATG4 Autophagy Related 4 Homolog B
Alternate Names
Apg4B, AUTL1
Gene Symbol
ATG4B
Additional ATG4B Products
Product Documents for ATG4B Antibody - BSA Free
Product Specific Notices for ATG4B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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