ATG4D Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92681

Novus Biologicals, part of Bio-Techne
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NBP2-92681-0.02ml
NBP2-92681-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATG4D Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 265-474 of human ATG4D (NP_116274.3). DVTRLVVYVSQDCTVYKADVARLVARPDPTAEWKSVVILVPVRLGGETLNPVYVPCVKELLRCELCLGIMGGKPRHSLYFIGYQDDFLLYLDPHYCQPTVDVSQADFPLESFHCTSPRKMAFAKMDPSCTVGFYAGDRKEFETLCSELTRVLSSSSATERYPMFTLAEGHAQDHSLDDLCSQLAQPTLRLPRTGRLLRAKRPSSEDFVFL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATG4D Antibody - BSA Free

Western Blot: ATG4D AntibodyBSA Free [NBP2-92681]

Western Blot: ATG4D AntibodyBSA Free [NBP2-92681]

Western Blot: ATG4D Antibody [NBP2-92681] - Analysis of extracts of various cell lines, using ATG4D at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: ATG4D Antibody - BSA Free [NBP2-92681]

Immunocytochemistry/ Immunofluorescence: ATG4D Antibody - BSA Free [NBP2-92681]

Immunocytochemistry/Immunofluorescence: ATG4D Antibody [NBP2-92681] - Analysis of U2OS cells using ATG4D at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for ATG4D Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATG4D

ATG4D is a gene that codes for a protein expressed in the skeletal muscle and testis and is 474 amino acids long with a weight of approximately 53 kDa. ATG4D is a cysteine protease required for autophagy. ATG4D has been shown to have interactions with TAF12, CASP3, CASP8, GABARAP, and UBC in pathways such as the regulation of autophagy pathway.

Alternate Names

APG4 autophagy 4 homolog D, APG4 autophagy 4 homolog D (S. cerevisiae), APG4D, APG4-D, ATG4 autophagy related 4 homolog D (S. cerevisiae), AUTL4, AUT-like 4 cysteine endopeptidase, AUT-like 4, cysteine endopeptidase, AUT-like 4, cysteine endopeptidase (S. cerevisiae), Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, cysteine protease ATG4D, cysteine protease involved in autophagy, EC 3.4.22, EC 3.4.22.-

Gene Symbol

ATG4D

Additional ATG4D Products

Product Documents for ATG4D Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATG4D Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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