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ATG9A Antibody (Atg9 14F2 8B1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50109

Novus Biologicals, part of Bio-Techne
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NBP2-50109-0.025ml

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Hamster IgG Clone # Atg9 14F2 8B1

Format

BSA Free

Concentration

0.7 mg/ml

Product Summary for ATG9A Antibody (Atg9 14F2 8B1) - BSA Free

Immunogen

Conserved C-terminal synthetic peptide of Human ATG9A sequence

Clonality

Monoclonal

Host

Hamster

Isotype

IgG

Theoretical MW

105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATG9A Antibody (Atg9 14F2 8B1) - BSA Free

Western Blot: ATG9A Antibody (Atg9 14F2 8B1)BSA Free [NBP2-50109]

Western Blot: ATG9A Antibody (Atg9 14F2 8B1)BSA Free [NBP2-50109]

Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of Hek293 lysates, non-transfected (nt) or transfected (trans) with HA-tagged human Atg9. Arrowhead indicates position of human Atg9 at 105 KDa.
Immunocytochemistry/ Immunofluorescence: ATG9A Antibody (Atg9 14F2 8B1) - BSA Free [NBP2-50109]

Immunocytochemistry/ Immunofluorescence: ATG9A Antibody (Atg9 14F2 8B1) - BSA Free [NBP2-50109]

Immunocytochemistry/Immunofluorescence: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Staining of PFA fixed HEK293 cells labelled with hamster anti-Atg9 (Left) and rabbit anti-Atg9.
Western Blot: ATG9A Antibody (Atg9 14F2 8B1)BSA Free [NBP2-50109]

Western Blot: ATG9A Antibody (Atg9 14F2 8B1)BSA Free [NBP2-50109]

Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of siRNA knockdown of human Atg9 (si) in Hela and Hek293 cells compared to siControl siRNA (siC) reveals specicity of hamster anti-Atg9 antibody.

Applications for ATG9A Antibody (Atg9 14F2 8B1) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): Endogenous protein, tagged over expressed protein and sRNA-depleted HEK 293 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

0.7 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG9A

Survival of environmental stress conditions requires the maintenance of cellular homeostasis. To preserve this balance, cells utilize a degradative mechanism known as autophagy. During this process, in response to starvation or other stresses, bulk cytoplasm is non-specifically sequestered within double-membrane vesicles and delivered to the lysosome/vacuole for subsequent degradation and recycling. Atg9 is the only integral membrane protein required for autophagosome formation and is considered a membrane carrier in autophagy-related pathways.

Alternate Names

APG9 autophagy 9-like 1, APG9 autophagy 9-like 1 (S. cerevisiae), APG9L1APG9-like 1, ATG9 autophagy related 9 homolog A (S. cerevisiae), autophagy 9-like 1 protein, autophagy-related protein 9A, FLJ22169, mATG9, MGD3208

Gene Symbol

ATG9A

Additional ATG9A Products

Product Documents for ATG9A Antibody (Atg9 14F2 8B1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATG9A Antibody (Atg9 14F2 8B1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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