Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ATG9B Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ATG9B. The immunogen is located within amino acids 850 - 900 of ATG9B. Amino Acid Squence: CPSQPPSPDEEKPSWSSD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

101 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATG9B Antibody - BSA Free

Western Blot: ATG9B AntibodyBSA Free [NBP1-77169]

Western Blot: ATG9B AntibodyBSA Free [NBP1-77169]

Western Blot: ATG9B Antibody [NBP1-77169] - HeLa cell lysate with ATG9B antibody at (A) 1 and (B) 2 ug/mL.
ATG9B Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ATG9B Antibody - BSA Free [NBP1-77169] -

Immunocytochemistry/ Immunofluorescence: ATG9B Antibody - BSA Free [NBP1-77169] - Immunofluorescence of ATG9B in Hela cells with ATG9B antibody at 20 u/mL.
ATG9B Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ATG9B Antibody - BSA Free [NBP1-77169] -

Immunocytochemistry/ Immunofluorescence: ATG9B Antibody - BSA Free [NBP1-77169] - Immunocytochemistry of ATG9B in HeLa cells with ATG9B antibody at 10 u/mL.

Applications for ATG9B Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG9B

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9B plays a role in autophagy and it's highly expressed in placenta and pituitary gland.

Alternate Names

APG9L2APG9-like 2, ATG9 autophagy related 9 homolog B (S. cerevisiae), autophagy-related protein 9B, endothelial nitric oxide synthase antisense, nitric oxide synthase 3 antisense, Nitric oxide synthase 3-overlapping antisense gene protein, NOS3ASautophagy 9-like 2 protein, Protein sONE, SONEFLJ14885

Entrez Gene IDs

285973 (Human); 213948 (Mouse); 499973 (Rat)

Gene Symbol

ATG9B

UniProt

Q674R7 (Human)

Additional ATG9B Products

Product Documents for ATG9B Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATG9B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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