ATP Citrate Lyase Antibody (3A5)

Western Blot: ATP Citrate Lyase Antibody (3A5) [NBP3-26530] -

Western Blot: ATP Citrate Lyase Antibody (3A5) [NBP3-26530] - Positive Western Blot detected in: L02 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, A549 whole cell lysate.
All lanes: ATP Citrate Lyase Antibody at 1: 1500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 121, 120, 92 kDa
Observed band size: 120 kDa

Immunocytochemistry/Immunofluorescence: ATP Citrate Lyase Antibody (3A5) [NBP3-26530] -

Immunocytochemistry/Immunofluorescence: ATP Citrate Lyase Antibody (3A5) [NBP3-26530] - Staining of HepG2 Cells with ATP Citrate Lyase Antibody (3A5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: ATP Citrate Lyase Antibody (3A5) [NBP3-26530] -

Flow Cytometry: ATP Citrate Lyase Antibody (3A5) [NBP3-26530] - Overlay histogram showing Hela cells stained with ATP Citrate Lyase Antibody (3A5) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.