Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3G8

Concentration

0.58 mg/ml

View all ATP Citrate Lyase Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ACLY (NP_001087) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

120.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATP Citrate Lyase Antibody (OTI3G8)

Western Blot: ATP Citrate Lyase Antibody (OTI3G8) [NBP2-02410]

Western Blot: ATP Citrate Lyase Antibody (OTI3G8) [NBP2-02410]

Western Blot: ATP Citrate Lyase Antibody (OTI3G8) [NBP2-02410] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP Citrate Lyase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP Citrate Lyase.
Immunocytochemistry/ Immunofluorescence: ATP Citrate Lyase Antibody (OTI3G8) [NBP2-02410]

Immunocytochemistry/ Immunofluorescence: ATP Citrate Lyase Antibody (OTI3G8) [NBP2-02410]

Immunocytochemistry/Immunofluorescence: ATP Citrate Lyase Antibody (OTI3G8) [NBP2-02410] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP Citrate Lyase.
Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (OTI3G8) [NBP2-02410]

Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (OTI3G8) [NBP2-02410]

Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (OTI3G8) [NBP2-02410] - Staining of paraffin-embedded Kidney tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50.
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Applications for ATP Citrate Lyase Antibody (OTI3G8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.58 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATP Citrate Lyase

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

Long Name

ATP-citrate synthase

Alternate Names

ACLY, EC 2.3.3.8

Entrez Gene IDs

47 (Human)

Gene Symbol

ACLY

Additional ATP Citrate Lyase Products

Product Documents for ATP Citrate Lyase Antibody (OTI3G8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATP Citrate Lyase Antibody (OTI3G8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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